Effects of fatty acids and growth hormone on liver fatty acid binding protein and PPARa in rat liver

نویسندگان

  • LINDA CARLSSON
  • DANIEL LINDÉN
  • MASOUMEH JALOULI
  • Daniel Lindén
  • Masoumeh Jalouli
چکیده

Carlsson, Linda, Daniel Lindén, Masoumeh Jalouli, and Jan Oscarsson. Effects of fatty acids and growth hormone on liver fatty acid binding protein and PPARa in rat liver. Am J Physiol Endocrinol Metab 281: E772–E781, 2001.—The aim of this study was to investigate the interaction between long-chain fatty acids (LCFA) and growth hormone (GH) in the regulation of liver fatty acid binding protein (LFABP) and peroxisome proliferator-activated receptor-a (PPARa). Cultured rat hepatocytes were given oleic acid (OA; 500 mM) and GH (100 ng/ml) for 3 days. LFABP mRNA increased 3.6-fold by GH and 5.7-fold by OA, and combined incubation with GH and OA increased LFABP mRNA 17.6-fold. PPARa mRNA was decreased 50% by GH, but OA had no effect. Hypophysectomized (Hx) female rats were treated with L-thyroxine, cortisol, GH, and dietary fat for 7 days. PPARa mRNA levels were threeto fourfold higher in Hx than in normal female rats. GH decreased PPARa mRNA 50% in Hx rats. Dietary triglycerides (10% corn oil) increased LFABP mRNA and cytosolic LFABP about twofold but had no effect on PPARa mRNA in Hx rats. GH and dietary triglycerides had an additive effect on LFABP expression. Dietary triglycerides increased mitochondrial hydroxymethylglutaryl-CoA synthase mRNA only in the presence of GH. The diet increased serum triglycerides in Hx rats, and GH treatment prevented this increase. Addition of cholesterol to the diet did not influence LFABP levels but mitigated increased hepatic triglyceride content. In summary, these studies show that GH regulates LFABP expression independently of PPARa. Moreover, GH has different effects on PPARa-responsive genes and does not counteract the effect of LCFA on the expression of these gene products.

منابع مشابه

Effects of feed restriction and dietary fat type on mRNA expression of liver fatty acid-binding protein (L-FABP) in broilers

Background: Liver fatty acid-binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance the uptake of fatty acids into the cell by increasing their concentration due to decreasing concentration of unbound fatty acids inside the cell. Objectives: The aim of this study was to evaluate the effects of dietary unsaturated to saturated fatty a...

متن کامل

پاسخ متفاوت سلولهای قلبی به اسیدهای چرب اشباع و غیر اشباع

Introduction & Objective: The link between dietary fat and coronary heart disease has attracted much attention since the effect of long?chain fatty acids (LCFA) on gene transcription has been established, which in part, these effects can be explained by the regulation of gene transcription. In this study, the P19CL6 cardiac cell?line was targeted for the investigation of (i) the effects of long...

متن کامل

Effects of Ramadan fasting on plasma free fatty acids in patients with non-alcoholic fatty liver disease

Introduction: Nonalcoholic fatty liver disease (NAFLD) is a global disease which its prevalence is about 10-35%. Several factors are involved in the pathogenesis of the disease. The present study was conducted to evaluate the effect of fasting during Ramadan on plasma free fatty acids in patients with NAFLD.Methods: This cross-sectional study was performed during the month of Ramadan in June-Ju...

متن کامل

Impacts of different dietary lipid sources on growth performance, fatty acid composition and antioxidant enzyme activity of juvenile Black Sea bream, Acanthopagrus schlegeli

The aim of the present study was to evaluate the impacts of diets supplemented with different lipid sources on growth performance, body composition, fatty acid profile, and hepatic antioxidant enzyme activity of juvenile black sea bream, Acanthopagrus schlegeli (initial mean weight, 1.1±0.02g). Four isonitrogenous and isolipidic diets were formulated with either fish oil (FO), soybean oil (SO),...

متن کامل

Seasonal variations in the fatty acid profiles of the liver and muscle of Squalius cephalus (Teleostei: Cyprinidae) living in Tödürge Lake (Sivas, Turkiye)

In this study, seasonal variations in the fatty acid profiles of the liver and muscle of Squalius cephalus from Tödürge Lake were determined by Gas Chromatography (GC). C22:6 n-3, C20:5 n-3, C20:4 n-6, C18:1 n-9, C18:1 n-7, C16:1 n-7, C18:0 and C16:0 were the principal fatty acids showing the highest levels in the muscle and liver of Squalius cephalus in all seasons. Although C22:6 n-3, which i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

متن کامل
عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2001